Comparative Proteomics and Difference Gel Electrophoresis

The goal of comparative proteomic is to analyze proteome changes in response to development, disease, or environment. This is a two step process in which proteins within cellular extracts are first fractionated to reduce sample complexity and then the proteins are identified by mass spectrometry. Two-dimensional electrophoresis (2DE) is the long times standard for protein separation, but it has suffered from poor reproducibility and limited sensitivity, Difference gel electrophoresis (DIGE), in which two protein samples are separately labeled with different fluorescent dyes and then co-electrophoresed on the same 2DE gel, was developed to overcome the reproducibility and sensitivity limitation. In this essay-I discuss the principles of comparatives proteomics and the development of DIGE.