Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 306, Issue 1-2, Pages 87-94Publisher
SPRINGER
DOI: 10.1007/s11010-007-9557-8
Keywords
Advanced glycation end products; RAGE; galectin-3; osteoblasts; apoptosis; AGE-receptors; regulation
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Funding
- NIGMS NIH HHS [R01 GM070589-01] Funding Source: Medline
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Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients.
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