Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 104, Issue 6, Pages 517-520Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1263/jbb.104.517
Keywords
Pseudozyma; yeast; transformation; electroporation; green fluorescent protein (GFP); hygromycin B
Ask authors/readers for more resources
A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/mu g of plasmid DNA. Transformants of Pseudozyma antarctica T-34 expressing a green fluorescent protein were obtained by the procedure.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
