Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 389, Issue 7-8, Pages 2097-2102Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-007-1627-y
Keywords
lectin; pseudo-lectin; affinity chromatography; electrophoresis
Ask authors/readers for more resources
We introduce a novel combination of boronic acid affinity chromatography with lectin affinity chromatography, dubbed as boronic acid-lectin affinity chromatography (BLAC). Concanavalin A and wheat germ agglutinin lectins were mixed with the pesudo-lectin boronic acid to form the BLAC affinity column and their performance was evaluated with standard glycoproteins. Optimization of the binding and elution buffers for the BLAC system is described. The BLAC columns were employed to isolate glycoproteins of interest using both selective and/or combined elution.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available