Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 468, Issue 1, Pages 114-120Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2007.09.018
Keywords
12-HETE; intramitochondrial ionized calcium; mtNOS; mitochondrial respiration; mitochondrial transmembrane potential; peroxynitrite; cytochrome c release; apoptosis
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Funding
- NIA NIH HHS [R03 AG023264-01A1, R03 AG023264-03, R03 AG023264, R03 AG023264-02, R03 AG023264-04] Funding Source: Medline
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12(S)-Hydroxyeicosatetraenoic acid (12-HETE) is one of the metabolites of arachidonic acid involved in pathological conditions associated with mitochondria and oxidative stress. The present study tested effects of 12-HETE on mitochondrial functions. In isolated rat heart mitochondria, 12-HETE increases intramitochondrial ionized calcium concentration that stimulates mitochondrial nitric oxide (NO) synthase (mtNOS) activity. mtNOS-derived NO causes mitochondrial dysfunctions by decreasing mitochondrial respiration and transmembrane potential. mtNOS-derived NO also produces peroxynitrite that induces release of cytochrome c and stimulates aggregation of mitochondria. Similarly, in HL-1 cardiac myocytes, 12-HETE increases intramitochondrial calcium and mitochondrial NO, and induces apoptosis. The present study suggests a novel mechanism for 12-HETE toxicity. (C) 2007 Elsevier Inc. All rights reserved.
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