Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source

We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image. (C) 2007 Optical Society of America.