Journal
CRYOBIOLOGY
Volume 55, Issue 3, Pages 222-229Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2007.08.006
Keywords
vitrification; 1,2-propanediol; sucrose; quartz microcapillary; open pulled straw; slush nitrogen; oocyte; ultrafast cooling rates
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Funding
- NIBIB NIH HHS [EB002340] Funding Source: Medline
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In this paper we report the thermal behavior of a new approach for vitrification. Thermal performance of traditional open pulled straws is compared with a new technique based on the combined use of quartz capillaries with slush nitrogen. This new method of vitrification achieved ultrafast cooling rates of 250,000 degrees C/min. As a result, a much lower concentration of cryoprotectant was needed to reach vitrification. In fact, a cryoprotectant solution typically used in oocyte slow freezing protocols was shown to remain transparent after cooling to liquid nitrogen temperatures indicating apparent vitrification. This approach offers a new and very promising technique for vitrification of cells using low levels of cryoprotectants. (C) 2007 Elsevier Inc. All rights reserved.
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