Journal
INSECT MOLECULAR BIOLOGY
Volume 16, Issue 6, Pages 753-759Publisher
WILEY
DOI: 10.1111/j.1365-2583.2007.00768.x
Keywords
innate immunity; NF-kappa B-like transcription factor; IMD pathway; RNA interference; quantitative PCR
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Relationships of immune genes in adult honeybees (Apis mellifera) were investigated using RNA interference (RNAi). Quantitative RT-PCR was applied to estimate gene expression and the extent of gene silencing. Relish is a transcription factor and forms an important part of the IMD signalling pathway. The expression of the immune gene Relish was significantly reduced by RNAi (ca. 70%). The proposed regulation of antimicrobial peptide genes by Relish could be established for abaecin and hymenoptaecin. These two genes showed a reduction in gene expression to the same extent as Relish. However, the antimicrobial peptide gene defensin-1 was not affected which suggests defensin-1 is regulated by a different signalling pathway.
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