Journal
INSECT SCIENCE
Volume 14, Issue 6, Pages 443-449Publisher
WILEY
DOI: 10.1111/j.1744-7917.2007.00172.x
Keywords
acetylcholinesterase; Bmace1; Bmace2; characterization; expression; silkworm
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Complementary DNAs encoding two types of acetylcholinesterase (AChE) were isolated from the silkworm, Bombyx mori. The type 1 (Bmace1) and type 2 (Bmace2) ORFs are 2052 and 1917 bp in length, respectively. Both the complete ORFs of the Bmaces and C-terminal truncated forms were recombined into the Bacmid baculovirus vector under the control of the polyhedrin promoter and expressed in Trichoplusia ni (Tn-5B1-4) cells. The resulting products exhibited AChE activity and glycosylation of the expressed proteins. An inhibition assay indicated that the ace2-type enzyme was more sensitive than the ace1-type enzyme to inhibition by eserine and paraoxon.
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