Journal
JOURNAL OF NEUROIMMUNOLOGY
Volume 192, Issue 1-2, Pages 49-56Publisher
ELSEVIER
DOI: 10.1016/j.jneuroim.2007.09.013
Keywords
ATP; neuro-immune cross-talk; Ca2+ signal; mast cells; neurites
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Previously, we showed that nerve-mast cell cross-talk can occur bidirectionally and that substance P is a mediator to activate mast cells. Here, we have studied the mediators to activate nerves cocultured with mast cells. Addition of antigen to the cocultures of superior cervical ganglia (SCG) and rat basophilic leukemia cells (RBLs) elicited Ca2+ response in RBLs and after a lag period induced Ca2+ signal in SCG neurites. Pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (purinergic receptor antagonist) or apyrase (ATP-hydrolyzing enzyme) reduced the Ca2+ signals in neurites, indicating that ATP released from activated rnast cells was one of important mediators to activate nerves. (c) 2007 Elsevier B.V. All rights reserved.
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