Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 328, Issue 1-2, Pages 215-219Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2007.09.004
Keywords
monocytes/macrophages; differentiation; bioassay; multi-well plate
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A new simple multi-well plate-based assay to determine monocyte differentiation-inducing activity was developed. It is based on the increase of macrophage adherence after the induction of monocyte differentiation. The assay was conducted using a human monocytic leukemia cell line, THP-1, treated with either 12-O-tetradecanoylphorbol 13-acetate or cytokines, such as tumor necrosis factor-alpha and interferon-gamma. This method is sensitive and easy to perform, especially in case of analyzing a large number of samples. (C) 2007 Elsevier B.V. All rights reserved.
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