Journal
JOURNAL OF PROTEOME RESEARCH
Volume 6, Issue 12, Pages 4601-4607Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr070401e
Keywords
comparative proteomics; O-18 labeling; reverse labeling; solution isoelectric focusing; galectin-3 binding protein; human mitochondria; drug resistance
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Funding
- NCI NIH HHS [R01 CA126189, CA 126189, R01 CA126189-01] Funding Source: Medline
- NIGMS NIH HHS [R37 GM021248, GM 21248, R01 GM021248, R01 GM021248-31] Funding Source: Medline
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Forward and reverse O-18 labeling are integrated with solution isoelectric focusing and capillary LC-tandem mass spectrometry to evaluate a new strategy for quantitative proteomics and to study abundance changes in mitochondrial proteins associated with drug resistance in MCF-7 human cancer cells. Galectin-3 binding protein, which is involved in apoptosis, was detected only in the resistant cell line, as a result of reverse labeling. Among 278 proteins identified, 12 were detected with abundances altered at least 2-fold.
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