Spectrofluorimetric determination of trace amounts of tyrosine with 1,5-bis (4,6-dicholro-1,3,5-triazinylamino) naphthalene

1, 5-Bis (4, 6-dicholro-1, 3, 5-triazinylamino) naphthalene was synthesized as a new fluorescent reagent. A novel fluorescent method was developed for the determination of tyrosine. Under pH = 12. 0, 35 degrees C for 30 min the fluorescent intensity at lambda(ex)/lambda(em) = 400 nm/465 nm was directly proportional to the concentration of tyrosine in the range of 1.1 x 10(-7) -1.1 x 10(-5) mol/L with the detection limit of 6.8 x 10(-8) mol/L. The proposed method has been successfully applied to the determination of trace amounts of tyrosine in human serum and urine samples.