Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 129, Issue 51, Pages 16042-16047Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja075715h
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Funding
- BBSRC [BB/D010918/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D010918/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/D001846/1] Funding Source: researchfish
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We form planar lipid bilayers between an aqueous droplet and a hydrogel support immersed in a lipid-oil solution. By scanning the bilayer over the surface of an SDS - PAGE gel, we are able to directly detect membrane proteins from gels using single-channel recording. Using this technique, we are able to examine low levels of endogenous protein from cell extracts without the need for over-expression. We also use droplet bilayers to detect small molecules from hydrogels. The bilayers show enhanced stability compared to conventional planar lipid bilayers, and both bilayer size and position can be controlled during an experiment. Hydrogel scanning with droplet bilayers provides a new method for the discovery and characterization of ion channels with the potential for high-throughput screening.
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