Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1176, Issue 1-2, Pages 217-222Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2007.11.013
Keywords
Cichorium glandulosum; HSCCC; lactucopicrin; 11 beta; 13-dihydrolactucin; lactucin
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Because of the skeletal complexity and similarity of the polarity, little research was reported on the isolation of sesquiterpene lactones by highspeed counter-current chromatography (HSCCC). Herein, three sesquiterpene lactones were successfully purified from the ethyl acetate extract of the roots of the traditional Uyghur medicinal plant Cichorium glandulosum Boiss. et Huet. by HSCCC. The separation was performed in two steps with two solvent systems: n-hexane-ethyl acetate-methanol-water (1.5:5:2.75:5, v/v/v/v) and ethyl acetate-methanol-water (20:1:20, v/v/v). From 166 mg of the ethyl acetate extract, 19 mg of lactucopicrin was isolated with the first solvent system and 10 mg of 11 beta, 13-dihydrolactucin and 16 mg of lactucin were obtained with the second solvent system. All purified compounds were over 94% purity as determined by HPLC analysis, and these chemical structures were confirmed by H-1 NMR and C-13 NMR. (c) 2007 Elsevier B.V. All rights reserved.
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