Determination of sex and gonadal maturity in the honeycomb grouper, Epinephelus merra, through biopsy

Determination of the sex and gonadal maturity of fish is necessary for successful commercial aquaculture operations. Sex identification is problematic in grouper fish, because they change sex at certain ages or sizes. Here we represent an accurate and reliable method for sex determination of the live grouper, using 5-10 mg gonadal tissue samples, harvested using non-lethal gonadal biopsy. Sex and sexual maturity of biopsied individuals were determined using standard histological method. All biopsied fish survived and there were no serious infections resulting from the surgery. Therefore, this biopsy technique is a simple and cost-effective tool for grouper fishery management.