Journal
BIOTECHNOLOGY LETTERS
Volume 30, Issue 2, Pages 243-252Publisher
SPRINGER
DOI: 10.1007/s10529-007-9526-z
Keywords
aptameric enzyme subunit; enzyme-inhibiting aptamer; homogeneous DNA sensing
Categories
Ask authors/readers for more resources
We have developed an aptameric enzyme subunit (AES) which can detect the DNA in a homogeneous solution. The AES is an artificial enzyme subunit composed of an enzyme-inhibiting aptamer bearing a target-molecule binding site. We connected a probe DNA to a thrombin-inhibiting aptamer at its 5' or 3' end. The inhibitory activity of the thrombin-inhibiting aptamer bearing the probe DNA decreased compared to that of the original aptamer; however, it recovered upon hybridization with the target DNA. Using this AES, we were able to detect target DNAs by measuring the thrombin activity in a homogeneous solution.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available