Journal
JOURNAL OF LEUKOCYTE BIOLOGY
Volume 83, Issue 2, Pages 314-324Publisher
WILEY
DOI: 10.1189/jlb.1007689
Keywords
alternative activation; classical activation; soluble egg antigen
Categories
Funding
- NIAID NIH HHS [T32 AI007413] Funding Source: Medline
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Macrophages play a pivotal role in innate and acquired immune responses to Schistosoma mansoni. Classical (M1) or alternative (M2) activation states of these cells further delineate their roles in tissue damage through innate immunity or fibrotic remodeling, respectively. In the present study, we addressed the following question: Does systemic Th2-type cytokine polarization evoked by S. mansoni affect macrophage differentiation and activation? To this end, we analyzed bone marrow-derived macrophages from mice with S. mansoni egg-induced pulmonary granulomas and unchallenged (or naive) mice to determine their activation state and their response to specific TLR agonists, including S. mansoni egg antigens. Unlike naive macrophages, macrophages from Th2-polarized mice constitutively expressed significantly higher found in inflammatory zone-1 (FIZZ1) and ST2 (M2 markers) and significantly lower NO synthase 2, CCL3, MIP-2, TNF-alpha, and IL-12 (M1 markers). Also, compared with naive macrophages, Th2-polarized macrophages exhibited enhanced responses to the presence of specific TLR agonists, which consistently induced significantly higher levels of gene and protein levels for M2 and M1 markers in these cells. Together, these data show that signals received by bone marrow precursors during S. mansoni egg-induced granuloma responses dynamically alter the development of macrophages and enhance the TLR responsiveness of these cells, which may ultimately have a significant effect on the pulmonary granulomatous response.
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