Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 390, Issue 3, Pages 841-846Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-007-1647-7
Keywords
microfluidics; planar bilayer; electrophysiology; membrane channel
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A microfluidic device was designed allowing the formation of a planar lipid bilayer across a micron-sized aperture in a glass slide sandwiched between two polydimethylsiloxane channel systems. By flushing giant unilamellar vesicles through a 500-mu m-wide channel above the hole, we were able to form a planar lipid bilayer across the hole, resulting in a giga-seal. We demonstrate incorporation of biological nanopores into the bilayer. This miniaturized system offers noise recordings comparable to open headstage noise (under 1 pA RMS at 10 kHz), fast precision perfusion on each side of the membrane and the use of nanoliter analyte volumes. This technique shows a promising potential for automation and parallelization of electrophysiological setups.
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