Journal
PHOTOSYNTHESIS RESEARCH
Volume 95, Issue 2-3, Pages 291-298Publisher
SPRINGER
DOI: 10.1007/s11120-007-9245-2
Keywords
bacterial photosynthesis; fluorescence; light-harvesting 2 complex; membrane reconstitution; phospholipids; purple bacteria; time-resolved spectroscopy
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Funding
- Biotechnology and Biological Sciences Research Council [BB/D000610/1] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/D000610/1] Funding Source: Medline
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Picosecond time-resolved fluorescence spectroscopy has been used in order to compare the fluorescence kinetics of detergent-solubilized and membrane-reconstituted light-harvesting 2 (LH2) complexes from the purple bacteria Rhodopseudomonas (Rps.) acidophila and Rhodobacter (Rb.) sphaeroides. LH2 complexes were reconstituted in phospholipid model membranes at different lipid:protein-ratios and all samples were studied exciting with a wide range of excitation densities. While the detergent-solubilized LH2 complexes from Rps. acidophila showed monoexponential decay kinetics (tau(f) = 980 ps) for excitation densities of up to 3.10(13) photons/(pulse.cm(2)), the membrane-reconstituted LH2 complexes showed multiexponential kinetics even at low excitation densities and high lipid:protein-ratios. The latter finding indicates an efficient clustering of LH2 complexes in the phospholipid membranes. Similar results were obtained for the LH2 complexes from Rb. sphaeroides.
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