Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 390, Issue 4, Pages 1093-1099Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-007-1773-2
Keywords
aptamers; fluorescence assays; thiazole orange; displacement assays
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We screened a series of RNA and DNA aptamers for their ability to serve in the dye displacement assays in which analytes compete with TO dye. We conclude that, while the performance of the TO dye displacement approach is not always predictable, it is still a simple and sensitive assay to detect binding between RNA aptamers and small molecules. In particular, we describe efficient assays for tobramycin and theophylline, with up to 90% displacement of TO observed, and we describe the first aptameric assay for cAMP.
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