Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 31, Issue 2, Pages 145-152Publisher
SPRINGER
DOI: 10.1007/s00449-007-0157-5
Keywords
software sensor; oxygen transfer; biomass; growth rate; stress; process analytical technology (PAT)
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Four software sensors based on standard on-line data from fermentation processes and simple mathematical models were used to monitor a number of state variables in Escherichia coli fed-batch processes: the biomass concentration, the specific growth rate, the oxygen transfer capacity of the bioreactor, and the new R-O/S sensor which is the ratio between oxygen and energy substrate consumption. The R-O/S variable grows continuously in a fed-batch culture with constant glucose feed, which reflects the increasing maintenance demand at declining specific growth rate. The R-O/S sensor also responded to rapid pH shift-downs reflecting the increasing demand for maintenance energy. It is suggested that this sensor may be used to monitor the extent of physiological stress that demands energy for survival.
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