Journal
ONCOGENE
Volume 27, Issue 7, Pages 896-906Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.onc.1210702
Keywords
Chk1; Chk2; DNA damage; G(2)/M checkpoint; mitosis; genome stability
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Whether Chk2 contributes to DNA damage- induced arrest in G(2) has been controversial. To investigate this issue further, we generated Chk2-deficient DT40 B-lymphoma cells by gene targeting and compared their cell cycle response to ionizing radiation (IR) with wildtype (WT) and isogenic Chk1-deficient counterparts. After moderate doses of IR (4 Gy), we find that Chk2(-/-) cells which are in G(1) or S phase at the time of irradiation arrest efficiently in G(2). In contrast, Chk2-/- cells which are in G(2) when DNA damage is incurred exhibit an impaired mitotic delay compared to WT, with the result that cells enter mitosis with damaged DNA as judged by the presence of numerous gamma-H2AX foci on condensed chromosomes. Impaired G(2) delay as the result of Chk2 deficiency can be detected at very low doses of radiation (0.1 Gy), and may allow division with spontaneous DNA damage, since a higher proportion of mitotic Chk2-/- cells bear spontaneous gamma-H2AX foci and damaged chromosomes during unperturbed growth compared to WT. The contribution of Chk2 to G(2)/M delay is epistatic to that of Chk1, since Chk1-/- cells exhibit no measurable mitotic delay at any radiation dose tested. We suggest that this function of Chk2 could contribute to tumour suppression, since cell division with low levels of spontaneous damage is likely to promote genetic instability and thus carcinogenesis.
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