Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 99, Issue 3, Pages 491-498Publisher
WILEY-BLACKWELL
DOI: 10.1002/bit.21713
Keywords
chloroperoxidase; Caldariomyces fumago; cultivation; microparticles; filamentous microorganisms
Categories
Ask authors/readers for more resources
Microparticle-enhanced cultivation (MPEC) was applied as a novel method for improved biomass and product formation during cultivation of filamentous microorganisms. Exemplarily, chloroperoxidase (CPO) formation by Caldariomyces fumago was analyzed in the presence and absence of microparticles of different size. Particles of similar to 500 mu m in diameter had no effect on growth morphology or productivity of CPO formation by C. fumago. In contrast particles of <= 42 mu m in diameter led to the dispersion of the C. fumago mycelia up to the level of single hyphae. Under these conditions the maximum specific productivity of CPO formation was enhanced about fivefold and an accumulated CPO activity in the culture supernatant of more than 1,000 U mL(-1) was achieved after 10-12 days of cultivation. In addition, the novel cultivation method also showed a positive effect on growth characteristics of other filamentous microorganisms proven by the stimulation of single hyphae/ cell formation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available