Journal
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
Volume 50, Issue 5, Pages 876-881Publisher
AMER THORACIC SOC
DOI: 10.1165/rcmb.2013-0166MA
Keywords
precision-cut lung slice; cryopreservation; airway contractility; airway smooth muscle
Funding
- National Institutes of Health [1R21HL112619]
- Parker Francis Foundation
- American Asthma Foundation
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An emerging tool in airway biology is the precision-cut lung slice (PCLS). Adoption of the PCLS as a model for assessing airway reactivity has been hampered by the limited time window within which tissues remain viable. Here we demonstrate that the PCLS can be frozen, stored long-term, and then thawed for later experimental use. Compared with the never-frozen murine PCLS, the frozen-thawed PCLS shows metabolic activity that is decreased to an extent comparable to that observed in other cryopreserved tissues but shows no differences in cell viability or in airway caliber responses to the contractile agonist methacholine or the relaxing agonist chloroquine. These results indicate that freezing and long-term storage is a feasible solution to the problem of limited viability of the PCLS in culture.
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