Journal
MOLECULAR IMAGING AND BIOLOGY
Volume 10, Issue 2, Pages 99-106Publisher
SPRINGER
DOI: 10.1007/s11307-007-0123-2
Keywords
epidermal growth factor receptor (EGFR); positron emission tomography (PET); heat shock protein 90 (Hsp90); 17-allyamino-17-demethoxygeldanamycin (17-AAG); cetuximab
Funding
- NCI NIH HHS [R24 CA93862, R21 CA102123, U54 CA119367, P50 CA114747] Funding Source: Medline
- NIBIB NIH HHS [R21 EB001785] Funding Source: Medline
Ask authors/readers for more resources
Purpose: The aim of this study is to non-invasively monitor the epidermal growth factor receptor (EGFR) response to a Hsp90 inhibitor-17-AAG treatment in a PC-3 prostate cancer model. Procedures: Nude mice bearing PC-3 tumor were injected intraperitoneally with 17-AAG and then imaged with micro positron emission tomography (microPET) using Cu-64-DOTA-cetuximab. Biodistribution studies, immunofluorescence staining, and Western blot were performed to validate the microPET results. Results: PC-3 cells are sensitive to 17-AAG treatment in a dose-dependent manner. Quantitative microPET showed that Cu-64-DOTA-cetuximab has prominent tumor activity accumulation in untreated tumors (14.6 +/- 2.6%ID/g) but significantly lower uptake in 17-AAG-treated tumors (8.9 +/- 1.6% ID/g) at 24 h post-injection. Both immunofluorescence staining and Western blot confirmed the significantly lower EGFR expression level in the tumor tissue upon 17-AAG treatment. Conclusions: The early response to anti-Hsp90 therapy was successfully monitored by quantitative PET using Cu-64-DOTA-cetuximab, which indicates that this approach may be valuable in monitoring the therapeutic response to Hsp90 inhibitor 17-AAG in EGFR-positive cancer patients.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available