Journal
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
Volume 45, Issue 4, Pages 809-816Publisher
AMER THORACIC SOC
DOI: 10.1165/rcmb.2010-0446OC
Keywords
mesenchymal stem cell; engraftment; lung resident; connexin 43; gap junctions
Funding
- NIH [RO1HL094622, R01 DK082481, RO1HL094311, RO1HL85149, RO1HL55397]
- American Thoracic Society
- Scleroderma Research Foundation
- Brian and Mary Campbell and Elizabeth Campbell Carr research gift fund
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Multipotent mesenchymal progenitor cells, termed mesenchymal stem cells (MSCs), have been demonstrated to reside in human adult lungs. However, there is little information regarding the associations of these local mesenchymal progenitors with other resident somatic cells and their potential for therapeutic use. Here we provide in vivo and in vitro evidence for the ability of human adult lung-resident MSCs (LR-MSCs) to interact with the local epithelial cells. The in vivo retention and localization of human LR-MSCs in an alveolar microenvironment was investigated by placing PKH-26 or DsRed lentivirus-labeled human LR-MSCs in the lungs of immunodeficient (SCID) mice. At 3 weeks after intratracheal administration, 19.3 +/- 3.21% of LR-MSCs were recovered, compared with 3.47 +/- 0.51% of control fibroblasts, as determined by flow cytometry. LR-MSCs were found to persist in murine lungs for up to 6months and demonstrated preferential localization to the corners of the alveoli in close proximity to type II alveolar epithelial cells, the progenitor cells of the alveolar epithelium. In vitro, LR-MSCs established gap junction communications with lung alveolar and bronchial epithelial cells and demonstrated an ability to secrete keratinocyte growth factor, an important modulator of epithelial cell proliferation and differentiation. Gap junction communications were also demonstrable between LR-MSCs and resident murine cells in vivo. This study demonstrates, for the first time, an ability of tissue-specific MSCs to engraft in their organ of origin and establishes a pathway of bidirectional interaction between these mesenchymal progenitors and adult somatic epithelial cells in the lung.
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