Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 56, Issue 3, Pages 275-283Publisher
HISTOCHEMICAL SOC INC
DOI: 10.1369/jhc.7A7310.2007
Keywords
collagen type XI; colon; immunohistochemistry; Golgi apparatus; 58K Golgi marker
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Funding
- NCRR NIH HHS [P20 RR016454-086155, P20 RR016454-096114, P20 RR016454, P-20RR-016454] Funding Source: Medline
- NIAMS NIH HHS [R01 AR047985-03, R01 AR047985-02, AR-47985, K02 AR048672-04, K02 AR048672-03, K02 AR048672-02, K02 AR048672-05, R01 AR047985, R01 AR047985-05, R01 AR047985-01, R01 AR047985-04, AR-48672, K02 AR048672-01, K02 AR048672] Funding Source: Medline
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In previous studies, collagen XI mRNA has been detected in colon cancer, but its location in human colon tissue has not been determined. The heterotrimeric collagen XI consists of three alpha chains. While it is known that collagen XI plays a regulatory role in collagen fibril formation, its function in the colon is unknown. The characterization of normal human colon tissue will allow a better understanding of the variance of collagen XI in abnormal tissues. Grossly normal and malignant human colon tissue was obtained from pathology archives. Immunohistochemical staining with a 58K Golgi markerand alpha 1(XI) and alpha 2(XI) antisera was used to specifically locate their presence in normal colon tissue. A comparative bright field microscopic analysis showed the presence of collagen XI in human colon. The juxtanuclear, dot-like collagenXI staining in the Golgi apparatus of goblet cells in normal tissue paralleled the staining of the 58K Golgi marker. Ultra light microscopy verified these results. Staining was also confirmed in malignant colon tissue. This study is the first to show that collagen XI is present in the Golgi apparatus of normal human colon goblet cells and localizes collagen XI in both normal and malignant tissue. Although the function of collagen XI in the colon is unknown, our immunohistochemical characterization provides the foundation for future immunohistopathology studies of the colon.
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