Journal
JOURNAL OF CLINICAL PATHOLOGY
Volume 61, Issue 3, Pages 343-346Publisher
B M J PUBLISHING GROUP
DOI: 10.1136/jcp.2007.050757
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Aims: To evaluate the accuracy of direct disc susceptibility testing performed from positive BACTEC blood culture vials, using a predetermined dilution protocol. Methods: Direct susceptibility testing was performed from 432 positive blood culture vials, generating 3829 antibiotic-organism results. Results were compared with those obtained by standard disc susceptibility testing according to Clinical Laboratory Standards Institute (CLSI) methods. Results: When results were compared with the reference method, no very major errors were detected. One (0.03%) major error and 89 (2.3%) minor errors were found. Error rates by organism group ranged from 1.3% for Pseudomonas aeruginosa to 8.2% for beta-haemolytic streptococci. Conclusions: Direct susceptibility testing provided accurate susceptibility results for most organism-antibiotic combinations, with the exception of the beta-haemolytic streptococci.
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