Journal
BIOTECHNOLOGY LETTERS
Volume 30, Issue 3, Pages 563-568Publisher
SPRINGER
DOI: 10.1007/s10529-007-9553-9
Keywords
catalase; E. coli; photoinhibition; rice; site directed mutagenesis
Categories
Ask authors/readers for more resources
Catalase plays a central role in plant stress responses but is highly susceptible to photoinhibition. A rice catalase-B protein avoiding photoinhibition was developed by mutagenesis of specific amino acids: Leu-189 to Trp-189 and His-225 to Thr-225 and then recombinantly expressed in E. coli. In addition, the site specific mutation also induced 2-2.5-fold increase in enzyme velocity with high affinity for its substrate and showed nearly a 3-fold lower K-m than the wild protein. These characteristic of mutated rice catalase-B is highly promising in transgenic research to increase plant productivity under stress conditions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available