Journal
PLANT CELL REPORTS
Volume 27, Issue 3, Pages 463-470Publisher
SPRINGER
DOI: 10.1007/s00299-007-0471-z
Keywords
agrobacterium transformation; dwarfism; gibberellin; gibberellin 2-oxidase; Phaseolus coccineus; Solanum melanocerasum; S. nigrum
Categories
Funding
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00004161, BBS/E/C/00004954, BBS/E/C/00004162] Funding Source: researchfish
- BBSRC [BBS/E/C/00004954] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00004161, BBS/E/C/00004162, BBS/E/C/00004954] Funding Source: Medline
Ask authors/readers for more resources
Gibberellins (GAs) are endogenous hormones that play a predominant role in regulating plant stature by increasing cell division and elongation in stem internodes. The product of the GA 2-oxidase gene from Phaseolus coccineus (PcGA2ox1) inactivates C-19-GAs, including the bioactive GAs GA(1) and GA(4), by 2 beta-hydroxylation, reducing the availability of these GAs in plants. The PcGA2ox1 gene was introduced into Solanum melanocerasum and S. nigrum (Solanaceae) by Agrobacterium-mediated transformation with the aim of decreasing the amounts of bioactive GA in these plants and thereby reducing their stature. The transgenic plants exhibited a range of dwarf phenotypes associated with a severe reduction in the concentrations of the biologically active GA(1) and GA(4). Flowering and fruit development were unaffected. The transgenic plants contained greater concentrations of chlorophyll b (by 88%) and total chlorophyll (11%), although chlorophyll a and carotenoid contents were reduced by 8 and 50%, respectively. This approach may provide an alternative to the application of chemical growth retardants for reducing the stature of plants, particularly ornamentals, in view of concerns over the potential environmental and health hazards of such compounds.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available