Journal
PHARMACEUTICAL RESEARCH
Volume 25, Issue 3, Pages 692-699Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11095-007-9282-8
Keywords
chondrogenesis; genetically engineered polymers; hydrogels; silk-elastinelike polymers; tissue engineering
Funding
- NIBIB NIH HHS [R01 EB003447-01A1, EB003447, R01 EB003447] Funding Source: Medline
Ask authors/readers for more resources
Purpose. In this study the chondrocytic differentiation and cartilage matrix accumulation of human mesenchymal stem cells (hMSCs) were investigated after encapsulation in a genetically engineered silk-elastinlike protein polymer SELP-47 K as an injectable matrix for delivery of cell-based therapeutics. Materials and Methods. hMSCs were encapsulated in SELP-47 K and cultured for 4 weeks in chondrogenic medium with or without transforming growth factor-beta 3 (TGF). Chondrogenic differentiation was evaluated by histological, RNA and biochemical analyses for the expression of cartilage extracellular matrix components. Results. Histological and immunohistochemical staining revealed that the cells acquired a rounded morphology and were embedded in significant amounts of chondrogenic extracellular matrix. Reverse transcriptase (RT)-PCR showed an up-regulation in aggrecan, type II and type X collagen and SOX9 in presence of TGF-beta 3. By day 28, constructs cultured in the presence of TGF-beta 3 exhibited significant increase in sulfated glycosaminoglycan and total collagen content up to 65 and 300%, respectively. Conclusions. This study demonstrates that SELP-47 K hydrogel can be used as a scaffold for encapsulation and chondrogenesis of hMSCs. The ability to use recombinant techniques to precisely control SELP structure enables the investigation of injectable protein polymer scaffolds for soft-tissue engineering with varied physicochemical properties.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available