Journal
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
Volume 70, Issue 6, Pages 464-471Publisher
WILEY
DOI: 10.1111/aji.12158
Keywords
Apoptosis; caspase3; signal transduction and activation of transcription 3; thymic stromal lymphopoietin; T cells
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Funding
- National Natural Science Foundation of China [31170870]
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ProblemTo investigate whether thymic stromal lymphopoietin (TSLP) regulates the apoptosis of decidual T cells and to elucidate the mechanism. Method of studyPrimary human decidual T cells were treated with TSLP only or TSLP combined with different signaling inhibitors (STAT3, STAT5, AKT, and ERK). The levels of signal transduction and activation of transcription 3 (STAT3) tyrosine phosphorylation and caspase3 expression were determined using Western blot analysis, and the apoptosis of decidual T cells was analyzed by flow cytometry. ResultsThe proportions of T cells in the peripheral circulation and in decidual CD3(+) cell population in women with normal pregnancy were higher than the proportions of T cells in either non-pregnant control or miscarriage. Decidual T cells co-expressed the TSLP receptors (TSLPR) and IL-7R, and the expression of TSLPR in decidual T cells was higher than that in decidual CD8(+) and CD4(+) T cells. Treatment with TSLP significantly suppressed the apoptosis of decidual T cells and enhanced STAT3 phosphorylation. Moreover, STAT3, and not other inhibitors, completely abrogated the anti-apoptotic effect and expression of caspase3 in decidual T cells induced by recombinant human TSLP. ConclusionThese results suggest that TSLP may down-regulate caspase3 expression through activation of the STAT3 pathway, thereby suppressing the apoptosis of decidual T cells.
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