Journal
ONCOGENE
Volume 27, Issue 12, Pages 1800-1804Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.onc.1210810
Keywords
HPV E6; p53; PDZ; E6AP; proteasome
Funding
- NCI NIH HHS [CA022443, CA098428] Funding Source: Medline
Ask authors/readers for more resources
Human papillomavirus(HPV) type 16 and 18 E6 proteins target many of their cellular substrates for proteasome-mediated degradation. In the case of p53, this is mediated by the E6AP ubiquitin ligase. However it is still unclear whether other E6 substrates, in particular those containing PDZ domains, are also degraded in a similar manner. To investigate this, we established an epithelial cell line from E6AP-null mice and used these cells as a background to perform E6-mediated in vivo degradation assays. We show that the PDZ domain-containing substrates of E6, including Scribble, MAGI-1 and MAGI-3, are all subject to E6-mediated degradation in these cells. Strikingly, we also found that p53 could be degraded by E6 within these cells in a proteasome-dependent manner. These results demonstrate that HPV-16 and -18 E6 can target substrates for degradation in a manner independent of the E6AP ubiquitin ligase.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available