Journal
AMERICAN JOURNAL OF PRIMATOLOGY
Volume 72, Issue 8, Pages 699-706Publisher
WILEY
DOI: 10.1002/ajp.20826
Keywords
pygmy loris; bacterial diversity; 16S rDNA; RFLP; phylogenetic analysis
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Funding
- National Natural Science Foundation of China [30960165]
- Key Project of the Yunnan Basic Applied Plan [2006C0004Z]
- Department of the Yunnan Province Development and Reform Commission [20081657]
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The bacterial diversity in fecal samples from the wild pygmy loris was examined with a 16S rDNA clone library and restriction fragment length polymorphism analysis. The clones were classified as Firmicutes (43.1%), Proteobacteria (34.5%), Actinobacteria (5.2%), and Bacteroidetes (17.2%). The 58 different kinds of 16S rDNA sequences were classified into 16 genera and 20 uncultured bacteria. According to phylogenetic analysis, the major genera within the Proteobacteria was Pseudomonas, comprising 13.79% of the analyzed clone sequences. Many of the isolated rDNA sequences did not correspond to known microorganisms, but had high homology to uncultured clones found in human feces. Am. J. Primatol. 72:699-706, 2010. (c) 2010 Wiley-Liss, Inc.
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