Journal
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
Volume 302, Issue 8, Pages R990-R995Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpregu.00659.2011
Keywords
collagen; cross-linking; hydroxylyslpyridinoline; hydroxyproline; stiffness
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Funding
- Midwestern University Office of Research and Sponsored Programs
- College of Health Sciences
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Carroll CC, Whitt JA, Peterson A, Gump BS, Tedeschi J, Broderick TL. Influence of acetaminophen consumption and exercise on Achilles tendon structural properties in male Wistar rats. Am J Physiol Regul Integr Comp Physiol 302: R990-R995, 2012. First published February 22, 2012; doi: 10.1152/ajpregu.00659.2011.-Chronic consumption of acetaminophen (APAP) during exercise training leads to a reduction in tendon stiffness and modulus compared with a placebo. We explored whether this effect could be due to a reduction in tendon collagen content or cross-linking. Ten-week-old male Wistar rats (n = 50) were divided into placebo or APAP groups and into sedentary or treadmill-exercised groups. APAP (200 mg/kg) or saline was administered once daily by oral gavage. Rats in the exercise groups ran on a treadmill 5 days per week for 8 wk with progression to 60 min per day, 20 m/min, and 8 degrees incline. After 8 wk, lyophilized Achilles tendon samples were assayed for the collagen-specific amino acid hydroxyproline and cross-linking [hydroxylyslpyridinoline (HP)] content by high-performance liquid chromatrography. Collagen content was not influenced by exercise or APAP (P > 0.05). Compared with placebo, tendon water content was 7% (P = 0.006, main effect) lower in animals consuming APAP (placebo: 54.79 +/- 0.8%, APAP: 50.89 +/- 1.2%). HP in the Achilles tendon was 36% greater (sedentary: 141 +/- 15, exercise: 204 +/- 26 mmol/mol collagen) in the exercise-trained rats independent of drug treatment (P = 0.020, main effect). Independent of exercise, HP content was 33% lower (P = 0.032, main effect) in the animals consuming APAP (placebo: 195 +/- 21, APAP: 140 +/- 19 mmol/mol collagen). Our data suggests that chronic consumption of APAP results in a reduction in collagen cross-linking and a loss of tissue water independent of chronic exercise. This reduction in cross-linking and water content could contribute to the decrease in tendon stiffness noted in humans chronically consuming APAP.
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