4.3 Article

Oral atorvastatin therapy increases nitric oxide-dependent cutaneous vasodilation in humans by decreasing ascorbate-sensitive oxidants

Publisher

AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpregu.00220.2011

Keywords

cholesterol; microvascular dysfunction; local heating; oxidant stress

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Funding

  1. National Institute of Health [R01-HL089302, M01-RR-10732]

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Holowatz LA, Kenney WL. Oral atorvastatin therapy increases nitric oxide-dependent cutaneous vasodilation in humans by decreasing ascorbate-sensitive oxidants. Am J Physiol Regul Integr Comp Physiol 301: R763-R768, 2011. First published June 29, 2011; doi:10.1152/ajpregu.00220.2011.-Elevated low-density lipoproteins (LDL) are associated with cutaneous microvascular dysfunction partially mediated by increased arginase activity, which is decreased following a systemic atorvastatin therapy. We hypothesized that increased ascorbate-sensitive oxidant stress, partially mediated through uncoupled nitric oxide synthase (NOS) induced by upregulated arginase, contributes to cutaneous microvascular dysfunction in hypercholesterolemic (HC) humans. Four microdialysis fibers were placed in the skin of nine HC (LDL = 177 +/- 6 mg/dl) men and women before and after 3 mo of a systemic atorvastatin intervention and at baseline in nine normocholesterolemic (NC) (LDL = 95 +/- 4 mg/dl) subjects. Sites served as control, NOS inhibited, L-ascorbate, and arginase-inhibited+L-ascorbate. Skin blood flow was measured while local skin heating (42 degrees C) induced NO-dependent vasodilation. After the established plateau in all sites, 20 mM ngname was infused to quantify NO-dependent vasodilation. Data were normalized to maximum cutaneous vascular conductance (CVC) (sodium nitroprusside + 43 degrees C). The plateau in vasodilation during local heating (HC: 78 +/- 4 vs. NC: 96 +/- 2% CVCmax, P < 0.01) and NO-dependent vasodilation (HC: 40 +/- 4 vs. NC: 54 +/- 4% CVCmax, P < 0.01) was reduced in the HC group. Acute L-ascorbate alone (91 +/- 5% CVCmax, P < 0.001) or combined with arginase inhibition (96 +/- 3% CVCmax, P < 0.001) augmented the plateau in vasodilation in the HC group but not the NC group (ascorbate: 96 +/- 2; combo: 93 +/- 4% CVCmax, both P > 0.05). After the atorvastatin intervention NO-dependent vasodilation was augmented in the HC group (HC postatorvastatin: 64 +/- 4% CVCmax, P < 0.01), and there was no further effect of ascorbate alone (58 +/- 4% CVCmax, P > 0.05) or combined with arginase inhibition (67 +/- 4% CVCmax, P > 0.05). Increased ascorbate-sensitive oxidants contribute to hypercholesteromic associated cutaneous microvascular dysfunction which is partially reversed with atorvastatin therapy.

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