Journal
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
Volume 300, Issue 5, Pages L790-L798Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajplung.00449.2010
Keywords
jugular ganglion; neurotrophin; brain-derived neurotrophic factor; glial cell-derived neurotrophic factor; tropomyosin receptor kinases a and b; glial cell-derive neurotrophic factor family receptor alpha 1
Categories
Funding
- National Institutes of Health, Bethesda, MD [R01HL038095, R01HL062296]
Ask authors/readers for more resources
Lieu T, Kollarik M, Myers AC, Undem BJ. Neurotrophin and GDNF family ligand receptor expression in vagal sensory nerve subtypes innervating the adult guinea pig respiratory tract. Am J Physiol Lung Cell Mol Physiol 300: L790-L798, 2011. First published February 18, 2011; doi:10.1152/ajplung.00449.2010.-We combined retrograde tracing techniques with single-neuron RT-PCR to compare the expression of neurotrophic factor receptors in nodose vs. jugular vagal sensory neurons. The neurons were further categorized based on location of their terminals (tracheal or lungs) and based on expression of the ionotropic capsaicin receptor TRPV1. Consistent with functional studies, nearly all jugular neurons innervating the trachea and lungs expressed TRPV1. With respect to the neurotrophin receptors, the TRPV1-expressing jugular C-fiber neurons innervating both the trachea and lung compartments preferentially expressed tropomyosin-receptor kinase A (TrkA), with only a minority of neurons expressing TrkB or TrkC. The nodose neurons that express TRPV1 (presumed nodose C-fibers) innervate mainly intrapulmonary structures. These neurons preferentially expressed TrkB, with only a minority expressing TrkA or TrkC. The expression pattern in tracheal TRPV1-negative neurons, nodose tracheal presumed A delta-fiber neurons as well as the intrapulmonary TRPV1-negative presumed A beta-fiber neurons, was similar to that observed in the nodose C-fiber neurons. We also evaluated the expression of GFR alpha receptors and RET (receptors for the GDNF family ligands). Virtually all vagal sensory neurons innervating the respiratory tract expressed RET and GFR alpha 1. The jugular neurons also categorically expressed GFR alpha 3, as well as similar to 50% of the nodose neurons. GFR alpha 2 was expressed in similar to 50% of the neurons irrespective of subtype. The results reveal that Trk receptor expression in vagal afferent neurons innervating the adult respiratory tract depends more on the location of the cell bodies (jugular vs. nodose ganglion) than either the location of the terminals or the functional phenotype of the nerve. The data also reveal that in addition to neurotrophins, the GDNF family ligands may be important neuromodulators of vagal afferent nerves innervating the adult respiratory tract.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available