Diabetes-induced vascular dysfunction involves arginase I

Romero MJ, Iddings JA, Platt DH, Ali MI, Cederbaum SD, Stepp DW, Caldwell RB, Caldwell RW. Diabetes-induced vascular dysfunction involves arginase I. Am J Physiol Heart Circ Physiol 302: H159-H166, 2012. First published November 4, 2011; doi: 10.1152/ajpheart. 00774.2011.-Arginase can cause vascular dysfunction by competing with nitric oxide synthase for L-arginine and by increasing cell proliferation and collagen formation, which promote vascular fibrosis/stiffening. We have shown that increased arginase expression/activity contribute to vascular endothelial cell (EC) dysfunction. Here, we examined the roles of the two arginase isoforms, arginase I and II (AI and AII, respectively), in this process. Experiments were performed using streptozotocin-induced diabetic mice: wild-type (WT) mice and knockout mice lacking the AII isoform alone (AI(+/+) AII(-/-)) or in combination with partial deletion of AI (AI(+/-) AII(-/-)). EC-dependent vasorelaxation of aortic rings and arterial fibrosis and stiffness were assessed in relation to arginase activity and expression. Diabetes reduced mean ECdependent vasorelaxation markedly in diabetic WT and AI(+/+) AII(-/-) aortas (53% and 44% vs. controls, respectively) compared with a 27% decrease in AI(+/-) AII(-/-) vessels. Coronary fibrosis was also increased in diabetic WT and AI(+/+) AII(-/-) mice (1.9-and 1.7-fold vs. controls, respectively) but was not altered in AI(+/-) AII(-/-) diabetic mice. Carotid stiffness was increased by 142% in WT diabetic mice compared with 51% in AI(+/+) AII(-/-) mice and 19% in AI(+/-) AII(-/-) mice. In diabetic WT and AI(+/+) AII(-/-) mice, aortic arginase activity and AI expression were significantly increased compared with control mice, but neither parameter was altered in AI(+/-) AII(-/-) mice. In summary, AI(+/-) AII(-/-) mice exhibit better EC-dependent vasodilation and less vascular stiffness and coronary fibrosis compared with diabetic WT and AI(+/+) AII(-/-) mice. These data indicate a major involvement of AI in diabetes-induced vascular dysfunction.