Journal
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
Volume 303, Issue 11, Pages E1363-E1372Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpendo.00199.2012
Keywords
cyclic nucleotide gated channels; insulin receptor; rod outer segments; phosphorylation; guanosine 3 ',5 '-cyclic monophosphate
Categories
Funding
- National Institutes of Health [EY-016507, EY-00871, EY-12190]
- NCRR COBRE [P20-RR-17703]
Ask authors/readers for more resources
Gupta VK, Rajala A, Rajala RV. Insulin receptor regulates photoreceptor cng channel activity. Am J Physiol Endocrinol Metab 303: E1363-E1372, 2012. First published October 2, 2012; doi:10.1152/ajpendo.00199.2012.-Photoreceptor cyclic nucleotide gated (CNG) channels are critical elements in phototransduction and light adaptation. Here we report that insulin receptor (IR), an integral membrane protein, directly phosphorylates the CNGA1 subunit of CNG channels that in turn affects the function of these channels negatively. The IR phosphorylates Tyr(498) and Tyr(503) residues on CNGA1 that are situated at the membrane-cytoplasmic interface. The IR tyrosine kinase activity is essential for the inhibition of CNG channel. To maintain the channels in an off state, it is necessary not only to have a precise balance of the cGMP levels but also to have a control on the cGMP sensitivity of the CNG channels itself. In this study, we observed that the channel opens at a lower concentration of cGMP in IR-/- mice. These studies suggest that IR regulates the modulation of CNG channel activity in vivo.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available