Journal
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
Volume 297, Issue 5, Pages E1154-E1161Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpendo.00086.2009
Keywords
microdialysis; adipose tissue; adipokines
Categories
Funding
- Grant Agency of the Czech Republic [GACR 303/04/0158]
- Ministry of Education of the Czech Republic [MSM 0021620814]
- MOLPAGE (Molecular Phenotyping to Accelerate Genomic Epidemiology)
- ADAPT [HEALTH-F2-2008-201100]
- European Union
Ask authors/readers for more resources
Siklova-Vitkova M, Polak J, Klimcakova E, Vrzalova J, Hejnova J, Kovacikova M, Kovacova Z, Bajzova M, Rossmeislova L, Hnevkovska Z, Langin D, Stich V. Effect of hyperinsulinemia and very-low-calorie diet on interstitial cytokine levels in subcutaneous adipose tissue of obese women. Am J Physiol Endocrinol Metab 297: E1154-E1161, 2009. First published September 1, 2009; doi: 10.1152/ajpendo.00086.2009.-Type 2 diabetes and obesity are associated with an enhanced release of a number of adipocytokines. Hyperinsulinemia, frequently present in type 2 diabetes and obesity, might be one of the drivers of the enhanced production of adipocytokines. The aim of this study was to investigate the interstitial levels of cytokines in subcutaneous adipose tissue (SCAT) in response to hyperinsulinemia and the effect of weight-reducing hypocaloric diet on this regulation in obese subjects. Thirteen obese premenopausal women participated in the study. Concentrations of seven cytokines were measured in plasma and in AT interstitial fluid collected by microdialysis during a euglycemic-hyperinsulinemic clamp and during control infusion of physiological saline. A subgroup of six women underwent a 4-wk very-low-calorie diet (VLCD). Microdialysis during the clamp was performed before and at the end of VLCD. Hyperinsulinemia induced an increase of monocyte chemoatractant protein (MCP-1) and IL-6 SCAT interstitial and plasma levels and elevated IL-8 levels in SCAT. The relative changes of IL-6 levels in the dialysate correlated with changes of IL-8 and MCP-1. The interstitial and plasma levels of IL-1 beta, IL-10, TNF alpha, and plasminogen activator inhibitor (PAI-1) remained unchanged in response to hyperinsulinemia. VLCD resulted in enhancement of the hyperinsulinemia-induced augmentation of MCP-1, IL-6, and IL-8 interstitial levels. In conclusion, hyperinsulinemia upregulates the interstitial levels of MCP-1, IL-6, and IL-8 in SCAT in obese women, whereas it does not affect IL-1 beta, IL-10, TNF alpha, and PAI-1 levels. Hypocaloric diet associated with weight reduction enhances the hyperinsulinemia-induced upregulation of MCP-1, IL-6, and IL-8 in SCAT.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available