Journal
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
Volume 305, Issue 11, Pages C1114-C1122Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpcell.00166.2013
Keywords
hyperosmotic saline; cystic fibrosis; Na+ transport; amiloride; human bronchial epithelial cells
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Funding
- Cystic Fibrosis Foundation
- Cystic Fibrosis Foundation Research Development Program
- National Institute of Diabetes and Digestive and Kidney Diseases [P30 DK-072506]
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Hypertonic saline (HS) inhalation therapy benefits cystic fibrosis (CF) patients [Donaldson SH, Bennet WD, Zeman KL, Knowles MR, Tarran R, Boucher RC. N Engl J Med 354: 241-250, 2006; Elkins MR, Robinson M, Rose BR, Harbour C, Moriarty CP, Marks GB, Belousova EG, Xuan W, Bye PT; the National Hypertonic Saline in Cystic Fibrosis (NHSCF) Study Group. N Engl J Med 354: 229-240, 2006]. Surprisingly, these benefits are long-lasting and are diminished by the epithelial Na+ channel blocker amiloride (Donaldson SH, Bennet WD, Zeman KL, Knowles MR, Tarran R, Boucher RC. N Engl J Med 354: 241-250, 2006). Our aim was to explain these effects. Human bronchial epithelial (hBE) cells from CF lungs were grown in inserts and were used in three experimental approaches: 1) Ussing chambers to measure amiloride-sensitive short-circuit currents (I-Na); 2) continuous perfusion Ussing chambers; and 3) near thin-film conditions in which the airway surface of the inserts was exposed to a small volume (30 mu l) of isosmotic or HS solution as the inserts were kept in their incubation tray and were subsequently used to measure I-Na under isosmotic conditions (near thin-film experiments; Tarran R, Boucher RC. Methods Mol Med 70: 479-492, 2002). HS solutions (660 mosmol/kgH(2)O) were prepared by adding additional NaCl to the isosmotic buffer. The transepithelial short-circuit current (I-SC), conductance (G(T)), and capacitance (C-T) were measured by transepithelial impedance analysis (Danahay H, Atherton HC, Jackson AD, Kreindler JL, Poll CT, Bridges RJ. Am J Physiol Lung Cell Mol Physiol 290: L558-L569, 2006; Singh AK, Singh S, Devor DC, Frizzell RA, van Driessche W, Bridges RJ. Methods Mol Med 70: 129-142, 2002). Exposure to apical HS inhibited I-Na, GT, and CT. The I-Na inhibition required 60 min of reexposure to the isosmotic solution to recover 75%. The time of exposure to HS required to inhibit I-Na was <2.5 min. Under near thin-film conditions, apical exposure to HS inhibited I-Na, but as osmotically driven water moved to the apical surface, the aqueous apical volume increased, leading to an amiloride-insensitive decrease in its osmolality and to recovery of I-Na that lagged behind the osmotic recovery. Amiloride significantly accelerated the recovery of I-Na following exposure to HS. Our conclusions are that exposure to HS inhibits hBE I-Na and that amiloride diminishes this effect.
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