Journal
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
Volume 299, Issue 1, Pages C33-C41Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpcell.00033.2010
Keywords
bicarbonate excretion; metabolic alkalosis; chloride excretion; cortical collecting duct
Categories
Funding
- Department of Veterans Affairs
- National Institute of Diabetes and Digestive and Kidney Diseases [DK-62809]
- DCA dialysis care group
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Amlal H, Petrovic S, Xu J, Wang Z, Sun X, Barone S, Soleimani M. Deletion of the anion exchanger Slc26a4 (pendrin) decreases apical Cl-/HCO3 exchanger activity and impairs bicarbonate secretion in kidney collecting duct. Am J Physiol Cell Physiol 299: C33-C41, 2010. First published April 7, 2010; doi:10.1152/ajpcell.00033.2010.-The anion exchanger Pendrin, which is encoded by SLC26A4 (human)/Slc26a4 (mouse) gene, is localized on the apical membrane of non-acid- secreting intercalated (IC) cells in the kidney cortical collecting duct (CCD). To examine its role in the mediation of bicarbonate secretion in vivo and the apical Cl-/HCO3- exchanger in the kidney CCD, mice with genetic deletion of pendrin were generated. The mutant mice show the complete absence of pendrin expression in their kidneys as assessed by Northern blot hybridization, Western blot, and immunofluorescence labeling. Pendrin knockout (KO) mice display significantly acidic urine at baseline [pH 5.20 in KO vs. 6.01 in wild type (WT); P < 0.0001] along with elevated serum HCO3 concentration (27.4 vs. 24 meq/l in KO vs. WT, respectively; P < 0.02), consistent with decreased bicarbonate secretion in vivo. The urine chloride excretion was comparable in WT and KO mice. For functional studies, CCDs were microperfused and IC cells were identified by their ability to trap the pH fluorescent dye BCECF. The apical Cl-/HCO3- exchanger activity in B-IC and non-A, non-B-IC cells, as assessed by intracellular pH monitoring, was significantly reduced in pendrin-null mice. The basolateral Cl /HCO3- exchanger activity in A-IC cells and in non-A, non-B-IC cells, was not different in pendrin KO mice relative to WT animals. Urine NH4+ (ammonium) excretion increased significantly, consistent with increased trapping of NH3 in the collecting duct in pendrin KO mice. We conclude that Slc26a4 (pendrin) deletion impairs the secretion of bicarbonate in vivo and reduces apical Cl-/HCO3- exchanger activity in B-IC and non-A, non-B-IC cells in CCD. Additional apical Cl-/HCO3- exchanger(s) is (are) present in the CCD.
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