Journal
AMERICAN JOURNAL OF PATHOLOGY
Volume 184, Issue 7, Pages 1981-1990Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ajpath.2014.03.017
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Funding
- Adaptable and Seamless Technology Transfer Program of the Japan Science and Technology Agency
- Uehara Memorial Foundation
- Intramural Program of the NM
- Center for Cancer Research, National Cancer Institute
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Our research group recently demonstrated that pericytes are major sources of the secreted glycoprotein and integrin ligand Lactadherin (MFG-E8) in B16 melanoma tumors, and that MFG-E8 promotes angiogenesis via enhanced PDGF-PDGFR beta signaling mediated by integrin-growth factor receptor crosstalk. However, sources of MFG-E8 and its possible roles in skin physiology are not well characterized. The objective of this study was to characterize the involvement of MFG-E8 in skin wound healing. In the dermis of normal murine and human skin, accumulations of MFG-E8 were found around CD31(+) blood vessels, and MFG-E8 colocalized with PDGFR beta(+), alpha SMA(+), and NG2(+) pericytes. MFG-E8 protein and mRNA levels were elevated in the dermis during full-thickness wound healing in mice. MFG-E8 was diffusely present in granulation tissue and was localized around blood vessels. Wound healing was delayed in MFG-E8 knockout mice, compared with the wild type, and myofibroblast and vessel numbers in wound areas were significantly reduced in knockout mice. Inhibition of MFG-E8 production with siRNA attenuated the formation of capillary-like structures in vitro. Expression of MFG-E8 in fibrous human granulation tissue with scant blood vessels was less than that in granulation tissue with many blood vessels. These findings suggest that MFG-E8 promotes cutaneous wound healing by enhancing angiogenesis.
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