Journal
AMERICAN JOURNAL OF PATHOLOGY
Volume 180, Issue 6, Pages 2249-2256Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ajpath.2012.02.032
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Funding
- U.S. Department of Energy, Office of Biological and Environmental Research and Low Dose Radiation [DE-AC02-05CH1123]
- National Cancer Institute (Bay Area Physical Sciences-Oncology Center, University of California, Berkeley, California) [R37CA064786, U54CA126552, R01CA057621, U54CA112970, U01CA143233, U54CA143836]
- U.S. Department of Defense [W81XWH0810736]
- Susan G. Komen Breast Cancer Foundation [02-1591, KG111229]
- NIH National Center for Research Resources [K26 RR024037]
- NIH National Cancer Institute [U01 CA14582]
- Lawrence Berkeley National Laboratory
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Intact organ structure is essential in maintaining tissue specificity and cellular differentiation. Small physiological or genetic variations lead to changes in microanatomy that, if persistent, could have functional consequences and may easily be masked by the heterogeneity of tissue anatomy. Current imaging techniques rely on histological, two-dimensional sections requiring sample manipulation that are essentially two dimensional. We have developed a method for three-dimensional imaging of whole-mount, unsectioned mammalian tissues to elucidate subtle and detailed micro- and macroanatomies in adult organs and embryos. We analyzed intact or dissected organ whole mounts with laser scanning-based tissue autofluorescence/fluorescence imaging (LS-TAFI). We obtained dear visualization of microstructures within murine mammary glands and mammary tumors and other organs without the use of immunostaining and without probes or fluorescent reporter genes. Combining autofluorescence with reflected light signals from chromophore-stained tissues allowed identification of individual cells within three-dimensional structures of whole-mounted organs. This technique could be useful for rapid diagnosis of human clinical samples and possibly the effect of subtle variations such as low dose radiation. (Am J Pathol 2012, 180:2249-2254 http://dx.doi.org/10.1016/j.ajpath.2012.02.032)
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