Journal
AMERICAN JOURNAL OF PATHOLOGY
Volume 178, Issue 5, Pages 2416-2423Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ajpath.2011.01.013
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Funding
- INSERM, ANR blanc [AO5120DD]
- European Grant Innochem [LSHB-CT-2005-518167]
- ANR Maladies Neurologiques et Psychiatriques [ANR-08-MNPS-003]
- ERC [ERC-2007 St.G. 210345]
- Assistance Publique-Hopitaux de Paris
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The pro-inflammatory cytokine IL-1 beta has been shown to promote angiogenesis. It can have a neurotoxic or neuroprotective effect. Here, we have studied the expression of IL-1 beta in vivo and the effect of the IL-1 receptor antagonist on choroidal neovascularization (CNV) and retinal degeneration (RD). IL-1 beta expression significantly increased after laser injury (real time PCR) in C57BL/6 mice, in the C57BL/6 Cx3cr1(-/-) model of age-related macular degeneration (enzyme-linked immunoabsorbent assay), and in albino Wistar rats and albino BALB Cx3cr1(+/+) and Cx3cr1(-/-) mice (enzyme-linked immunoabsorbent assay) after light injury. IL-1 beta was localized to Ly6G-positive, Iba1-negative infiltrating neutrophils in laser-induced CNV as determined by IHC. IL-1 receptor antagonist treatment significantly inhibited CNV but did not affect Iba1-positive macrophage recruitment to the injury site. IL-1 beta significantly increased endothelial cell outgrowth in aortic ring assay independently of vascular endothelial growth factor, suggesting a direct effect of IL-1 beta on choroidal endothelial cell proliferation. Inhibition of IL-1 beta in light- and laser-induced RD models did not alter photoreceptor degeneration in Wistar rats, C57BL/6 mice, or RD-prone Cx3cr1(-/-) mice. Our results suggest that IL-1 beta inhibition might represent a valuable and safe alternative to Inhibition of vascular endothelial growth factor in the control of CNV in the context of concomitant photoreceptor degeneration as observed in age-related macular degeneration. (AmJ Pathol 2011, 178:2416-2423; DOI: 10.1016/j.ajpath.2011.01.013)
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