Journal
AMERICAN JOURNAL OF PATHOLOGY
Volume 176, Issue 5, Pages 2352-2366Publisher
ELSEVIER SCIENCE INC
DOI: 10.2353/ajpath.2010.090839
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Funding
- Ministry of Education, Culture, Sports, Science and Technology [19689031]
- Special Coordination Funds for Promoting Science and Technology for Kyushu University
- research foundations from the general insurance association of Japan
- Takeda Science Foundation
- Astellas Foundation for Research on Metabolic Disorders
- Japan Orthopaedics and Traumatology foundation [0180]
- Grants-in-Aid for Scientific Research [19689031] Funding Source: KAKEN
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Traumatic injury in the central nervous system induces inflammation; however, the role of this inflammation is controversial. Precise analysis of the inflammatory cells is important to gain a better understanding of the inflammatory machinery in response to neural injury. Here, we demonstrated that leukotriene B4 plays a significant role in mediating leukocyte infiltration after spinal cord injury. Using flow cytometry, we revealed that neutrophil and monocyte/macrophage infiltration peaked 12 hours after injury and was significantly suppressed in leukotriene B4 receptor 1 knockout mice. Similar findings were observed in mice treated with a leukotriene B4 receptor antagonist. Further, by isolating each inflammatory cell subset with a cell sorter, and performing quantitative reverse transcription-PCR, we demonstrated the individual contributions of more highly expressed subsets, ie, interleukins 6 and 1 beta, tumor necrosis factor-alpha, and FasL, to the inflammatory reaction and neural apoptosis. Inhibition of leukotriene B4 suppressed leukocyte infiltration after injury, thereby attenuating the inflammatory reaction, sparing the white matter, and reducing neural apoptosis, as well as inducing better functional recovery. These findings are the first to demonstrate that leukotriene B4 is involved in the pathogenesis of spinal cord injury through the amplification of leukocyte infiltration, and provide a potential therapeutic strategy for traumatic spinal cord injury. (Am J Pathol 2010, 176:2352-2366 DOI: 10.2353/ajpath.2010.090839)
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