Journal
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
Volume 204, Issue 2, Pages -Publisher
MOSBY-ELSEVIER
DOI: 10.1016/j.ajog.2010.09.004
Keywords
expression; microRNA; placenta; preeclampsia
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Funding
- National Institute of Child Health and Human Development, National Institutes of Health [HD/HL R01-32562]
- March of Dimes [1 FY08-425]
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OBJECTIVE: The role of posttranscription regulation in preeclampsia is largely unknown. We investigated preeclampsia-related placental microRNA (miRNA) expression using microarray and confirmatory quantitative real-time polymerase chain reaction experiments. STUDY DESIGN: Placental expressions of characterized and novel miRNAs (1295 probes) were measured in samples collected from 20 preeclampsia cases and 20 controls. Differential expression was evaluated using Student t test and fold change analyses. In pathway analysis, we examined functions/functional relationships of targets of differentially expressed miRNAs. RESULTS: Eight miRNAs were differentially expressed (1 up-regulated and 7 down-regulated) among preeclampsia cases compared with controls. These included previously identified candidates (miR-210, miR-1, and a miRNA in the 14q32.31 cluster region) and others that are novel (miR-584 and miR-34c-5p). These miRNAs target genes that participate in organ/system development (cardiovascular and reproductive system), immunologic dysfunction, cell adhesion, cell cycle, and signaling. CONCLUSION: Expression of miRNAs that target genes in diverse pathophysiological processes is altered in the setting of preeclampsia.
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