Experimental amniotic fluid infection in sheep: Effects of Ureaplasma parvum serovars 3 and 6 on preterm or term fetal sheep

OBJECTIVE: The objective of the study was to determine the effects in late gestation of Ureaplasma parvum serovar 3 colonization and the effects, preterm, of U. parvum serovar 6. STUDY DESIGN: Ewes received an intraamniotic (IA) injection of U. parvum serovar 6 (20 x 106 colony-forming units [cfu]; n = 9), U. parvum serovar 3 (20 x 103 cfu; n = 6), vehicle (n = 10), or saline (n = 4) on day 80 of pregnancy ( d). The lambs were delivered at 125 d (U parvum serovar 6, n = 9; saline or media controls, n = 9) or 145 d (U parvum serovar 3, n = 6; media controls, n = 5) for assessment of inflammation and lung maturation. RESULTS: IA ureaplasmas caused histologic chorioamnionitis but not preterm delivery. Fetal lung epithelium was colonized with ureaplasmas at both gestational ages, and pulmonary interleukin-8 levels had doubled in the ureaplasma-colonized animals, compared with the controls at 145 d. Surfactant levels in bronchoalveolar lavage fluid had increased 8-fold and 2.5-fold at 125 and 145 d, respectively, after ureaplasma injection. CONCLUSION: Fetal lung inflammation and altered development accompanies ureaplasma colonization, regardless of age at delivery.