Journal
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
Volume 73, Issue 6, Pages 582-588Publisher
WILEY
DOI: 10.1111/aji.12367
Keywords
Exosomes; microvesicles; placenta; placenta perfusion; preeclampsia; pregnancy
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Funding
- German Research Foundation (Deutsche Forschungsgemeinschaft) [FI1635/1-1]
- Thuringian Ministry for Education, Science and Arts
- Abel Tasman Talent Program of the University of Groningen
- MRC [MR/J003360/1] Funding Source: UKRI
- Medical Research Council [MR/J003360/1] Funding Source: researchfish
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ProblemThe pregnancy-associated disease preeclampsia is related to the release of syncytiotrophoblast extracellular vesicles (STBEV) by the placenta. To improve functional research on STBEV, reliable and specific methods are needed to quantify them. However, only a few quantification methods are available and accepted, though imperfect. For this purpose, we aimed to provide an enzyme-linked sorbent assay (ELSA) to quantify STBEV in fluid samples based on their microvesicle characteristics and placental origin. Method of StudyEx vivo placenta perfusion provided standards and samples for the STBEV quantification. STBEV were captured by binding of extracellular phosphatidylserine to immobilized annexin V. The membranous human placental alkaline phosphatase on the STBEV surface catalyzed a colorimetric detection reaction. Results and ConclusionThe described ELSA is a rapid and simple method to quantify STBEV in diverse liquid samples, such as blood or perfusion suspension. The reliability of the ELSA was proven by comparison with nanoparticle tracking analysis.
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